deconvolution using metamorph software Search Results


laser scanning confocal microscope  (Yokogawa Electric)
99
Yokogawa Electric laser scanning confocal microscope
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Laser Scanning Confocal Microscope, supplied by Yokogawa Electric, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/laser scanning confocal microscope/product/Yokogawa Electric
Average 99 stars, based on 1 article reviews
laser scanning confocal microscope - by Bioz Stars, 2026-06
99/100 stars
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metamorph software version 6.3  (universal imaging inc)
90
universal imaging inc metamorph software version 6.3
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Metamorph Software Version 6.3, supplied by universal imaging inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/metamorph software version 6.3/product/universal imaging inc
Average 90 stars, based on 1 article reviews
metamorph software version 6.3 - by Bioz Stars, 2026-06
90/100 stars
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macro-program  (MetaMorph Inc)
90
MetaMorph Inc macro-program
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Macro Program, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/macro-program/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
macro-program - by Bioz Stars, 2026-06
90/100 stars
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axiovert 200 microscope  (Carl Zeiss)
90
Carl Zeiss axiovert 200 microscope
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Axiovert 200 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axiovert 200 microscope/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
axiovert 200 microscope - by Bioz Stars, 2026-06
90/100 stars
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axioplan 2 microscope  (Carl Zeiss)
90
Carl Zeiss axioplan 2 microscope
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Axioplan 2 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/axioplan 2 microscope/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
axioplan 2 microscope - by Bioz Stars, 2026-06
90/100 stars
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metamorph computer software  (MetaMorph Inc)
90
MetaMorph Inc metamorph computer software
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Metamorph Computer Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/metamorph computer software/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
metamorph computer software - by Bioz Stars, 2026-06
90/100 stars
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rolera em-c 2 camera  (QImaging)
90
QImaging rolera em-c 2 camera
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Rolera Em C 2 Camera, supplied by QImaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rolera em-c 2 camera/product/QImaging
Average 90 stars, based on 1 article reviews
rolera em-c 2 camera - by Bioz Stars, 2026-06
90/100 stars
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analysis program metamorph software  (MetaMorph Inc)
90
MetaMorph Inc analysis program metamorph software
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Analysis Program Metamorph Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/analysis program metamorph software/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
analysis program metamorph software - by Bioz Stars, 2026-06
90/100 stars
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software modules  (MetaMorph Inc)
90
MetaMorph Inc software modules
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Software Modules, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/software modules/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
software modules - by Bioz Stars, 2026-06
90/100 stars
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metamorph analysis software  (MetaMorph Inc)
90
MetaMorph Inc metamorph analysis software
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Metamorph Analysis Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/metamorph analysis software/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
metamorph analysis software - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
image analysis software  (MetaMorph Inc)
90
MetaMorph Inc image analysis software
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Image Analysis Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/image analysis software/product/MetaMorph Inc
Average 90 stars, based on 1 article reviews
image analysis software - by Bioz Stars, 2026-06
90/100 stars
  Buy from Supplier
metamorph imaging software  (MetaMorph Inc)
90
MetaMorph Inc metamorph imaging software
A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal <t>microscope</t> images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.
Metamorph Imaging Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal microscope images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.

Journal: Analytical chemistry

Article Title: A Novel Omniphobic Platform for Multicellular Spheroid Generation, Drug Screening, and On-Plate Analysis

doi: 10.1021/acs.analchem.1c01326

Figure Lengend Snippet: A) Representative confocal image of live (Calcein AM, green) and dead (Ethidium Homodimer, red) cells within 1000 OVCAR8 cells/20 μL spheroids generated on an omniphobic mesh plate, imaged on day 7. B) Quantification of live and dead cells within spheroids generated on the omniphobic mesh plate, from confocal images (two-tailed t-test, n>3, *p<0.0002). C) Representative phase images of spheroids on the omniphobic mesh plate (OMP) and 384-hanging drop plate (384-HDP) after 48 hours treatment with 10 μM of the anti-cancer drug paclitaxel. D) Quantification of percent viable cells within spheroids after paclitaxel treatment, as analyzed by resorufin fluorescence. The viability was normalized to control treatment with cell culture medium (one-way ANOVA, n>3, *p<0.0001). The omniphobic mesh plate demonstrated equivalent spheroid viability on the first (OMP-1) and fourth (OMP-4) use of a plate. E) Representative confocal microscope images of heterogeneous spheroids generated with 1:1 ratio of mTomato labeled Pt224 ovarian cancer stem cells (500 cells, CD133+ and ALDH+) and GFP labeled human embryonic kidney (HEK) cells (500 cells) on day 5. F) Graph representing viability of 1000 cells spheroids generated using OVCAR8 and OVCAR3-GFP cell lines, in response to 24 hr of chemotherapeutic drug (20 µM doxorubicin, 10 µM paclitaxel) across three different spheroid generating platforms: omniphobic mesh (OMP), 384 hanging drop plate (384-HDP) and commercially available, 96 well U-bottom plate (U-bottom). A significant difference in viability was observed (two way ANOVA, n=4, ****p< 0.0001) when comparing no treatment control to Doxorubicin and Paclitaxel across all platforms. Additionally, a significant difference was observed in (two way ANOVA, n=4, **** p<0.0001) OVCAR3-GFP OMP & HDP, compared to OVCAR3 U-bottom, (two way ANOVA, n=4, **** p<0.0001), and in OVCAR8 OMP compared to OVCAR8 U-bottom. There was also significant change (two way ANOVA, n=4, *** p<0.0008) OVCAR8 HDP compared to OVCAR8 U-bottom.

Article Snippet: The stained cells were carefully transferred to glass coverslips and imaged using a laser scanning confocal microscope (Olympus IX81, equipped with a Yokogawa CSU-X1 confocal scanning laser unit, Andor iXon x3 CCD camera, and Metamorph 7.8 software), with constant gain and exposure settings between samples.

Techniques: Generated, Two Tailed Test, Fluorescence, Control, Cell Culture, Microscopy, Labeling